Castoe Lab


Department of Biology

University of Texas Arlington

 

Castoe Lab Members

I am currently working on several projects that utilize RADseq and high-throughput sequencing in conjunction with traditional Sanger sequencing for phylogenetic, phylogeographic, and population genetic analysis. My dissertation research centers on population genetic variation in rattlesnake species to address questions related to population genetic structure, gene flow, local adaptation, and speciation. I’m also involved in a number of collaborative genomic and population genetic projects focused on diverse organisms, including Daphnia, Dipodomys, Micrurus, and Anolis.


Publications


Google Scholar Citation Link: http://scholar.google.com/citations?user=ugQddL4AAAAJ&hl=en


Andrew, A.L., B. Perry, D.C. Card, D.R. Schield, R.P. Ruggiero, S.E. McGaugh, A. Chowdhary, S.M. Secor, and T.A. Castoe. 2017. Growth and stress response mechanisms underlying post-feeding regenerative organ growth in the Burmese python. BMC Genomics 18:338.  PDF


Schield, D.R., R.H. Adams,  D.C. Card, B.W. Perry, G.I.M. Pasquesi, T. Jezkova, D.M. Portik, A.L. Andrew, C.L. Spencer, E.E. Sanchez, M.K. Fujita, S.P. Mackessy, and T.A. Castoe. 2017. Insight into the roles of selection in speciation from genomic patterns of divergence and introgression in secondary contact in venomous rattlesnakes. Ecology and Evolution Online Early: DOI 10.1002/ece3.2996. PDF


Adams, R.H., D.R. Schield, D.C. Card, H. Blackmon, and T.A. Castoe. 2017. GppFst: Genomic posterior predictive simulations of Fst and dXY for identifying outlier loci from population genomic data. Bioinformatics 33:1414-1415. PDF   see also Software Page (GppFst)


Shortt, J.A., D.C. Card, D.R. Schield, L. Yang, B. Zhong, T.A. Castoe, E.J. Carlton, and D.D. Pollock. 2017. Whole genome amplification and targeted genome sequencing in Shistosoma japonicum isolates. PLoS Neglected Tropical Diseases 11: e0005292. PDF


Streicher, J.W., J.P. McEntee, L. Drzich, D.C. Card, D.R. Schield, U. Smart, C.L. Parkinson, T. Jezkova, E.N. Smith, and T.A. Castoe. 2016. Genetic surfing, not
allopatric divergence, explains spatial sorting of mitochondrial haplotypes in highly venomous coralsnakes. Evolution 70:1435-1449. PDF  Supplementary Material   [Cover Article]



Card, D.C., D.R Schield, R.H. Adams, A.B. Corbin, B.W. Perrry, A.L. Andrew, G.I.M. Pasquesi, E.N. Smith, T. Jezkova, S.M. Boback, W. Booth, and T.A. Castoe. 2016. Phylogeographic and population genetic analyses reveal multiple species of Boa and independent origins of insular dwarfism. Molecular Phylogenetics and Evolution. PDF


Streicher, J.W., J.P. McEntee, L. Drzich, D.C. Card, D.R. Schield, U. Smart, C.L. Parkinson, T. Jezkova, E.N. Smith, and T.A. Castoe. 2016. Genetic surfing, not allopatric divergence, explains spatial sorting of mitochondrial haplotypes in highly venomous coralsnakes. Evolution. PDF


Adams. R.H., H.G. Blackmon, J. Reyes-Velasco, D.R. Schield, D.C. Card, A.L. Andrew, N. Waynewood, and T.A. Castoe. 2016. Microsatellite landscape evolutionary dynamics across 450 million years of vertebrate genome evolution. Genome 59:295-310 PDF


Schield, D.R., M.F. Walsh, D.C. Card,  A.L. Andrew, R.H. Adams, and T.A. Castoe. 2016. EpiRadSeq: genome-wide analysis of shifts in DNA methylation using next-generation s
equencing. Methods in Ecology and Evolution 7:60-69.  Cover Article PDF





Andrew, A.L., D.C. Card, R.P. Ruggiero, D.R. Schield, R.H. Adams, D.D. Pollock, S.M. Secor, and T.A. Castoe. 2015. Rapid changes in gene expression direct rapid shifts in intestinal form and function in the Burmese python after feeding. Physiological Genomics 47:147-157. PDF



Schield, D.R., D.C. Card, R.H. Adams, T. Jezkova, J. Reyes-Velasco, F.N. Proctor, C.L. Spencer, H.-W. Herrmann, S.P. Mackessy, and T.A. Castoe. 2015. Incipient speciation with biased gene flow between two lineages of the Western Diamondback Rattlesnake (Crotalus atrox). Molecular Phylogenetics and Evolution 83: 213-223. PDF


Jezkova, T., B.R. Riddle, D.C. Card, D.R. Schield, M.E. Eckstut, and T.A. Castoe. 2015. Genetic consequences of a post-glacial range expansion in two co-distributed rodents (genus Dipodemys) depend on ecology and genetic locus. Molecular Ecology 24: 83-97. PDF


Reyes-Velasco J., D.C. Card, A.L. Andrew, K.J. Shaney, R.H. Adams, D.R. Schield, N.R. Casewell, S.P. Mackessy, and T.A. Castoe. 2014. Expression of venom gene homologs in diverse python tissues suggests a new model for the evolution of snake venom. Molecular Biology and Evolution doi: 10.1093/molbev/msu294. PDF


Schield, D.R, D.C. Card, J. Reyes-Velasco, A.L. Andrew, C.A. Modahl, S.M. Mackessy, D.D. Pollock, and T.A. Castoe. In Press. A role for genomics in rattlesnake research – current knowledge and future potential. In: Rattlesnakes of Arizona, G.W. Schuett, L.W. Porras, and R.S. Reiserer (Eds.), Eco Books.


Card, D.C., D.R. Schield, J. Reyes-Velasco, A.L. Andrew, R.P. Ruggiero, D.D. Pollock, D.F. Tomback, S.J. Oyler-McCance, M.K. Fujita, and T.A. Castoe. 2014. Two low coverage bird genomes and a comparison of reference-guided versus de novo genome assemblies. PLoSOne 9: e106649. PDF


Shaney, K.J., D.R. Schield, D.C. Card, R.P. Ruggiero, D.D. Pollock. S.P. Mackessy, T.A. Castoe. In Press. Squamate reptile genomics and evolution. In: Handbook of Toxinology: Venom Genomics and Proteomics, P. Gopalakrishnakone (Ed.), Springer Reference Press, New York, NY.


Castoe. T.A., A.P.J. de Koning, K.T. Hall, D.C. Card, D.R. Schield, M.K. Fujita, R.P. Ruggiero, J.F. Degner, J.M. Daza, W. Gu, J. Reyes-Velasco, K.J. Shaney, J.M. Castoe, S.E. Fox, A.W. Poole, D. Polanco, J. Dobry, M.W.
Vandewege, Q. Li, R. Schott, A. Kapusta, P. Minx, C. Feschotte, P. Uetz, D.A. Ray, F.G. Hoffmann, R. Bogden, E.N. Smith, B.S.W. Chang, F. Vonk, N.R. Casewell, C.V. Henkel, M.K. Richardson, S.P. Mackessy, A.M. Bronikowski, M. Yandell, W.C. Warren, S.M. Secor, and D.D. Pollock. 2013. The Burmese Python genome reveals the molecular basis for extreme adaptation in snakes. Proceedings of the National Academy of Sciences, USA 110:20645-20650. PDF  Appendix  DataFile1  Datafile2  Datafile3


Schield, D.R. and H.L Waye. 2012. Thamnophis radix: tonic immobility. Natural History.     Herpetological Review 43(2): 352.


Recent Scientific Presentations and Published Abstracts

2016 Card, D.C.†, D.R. Schield, R.H. Adams, A.L. Andrew, B.W. Perry, F. Mazzotti, M.E. Hunter, K. Hart, & T.A. Castoe. Genome-wide evidence of evolution and adaptation in the invasive Florida python population. Joint Evolution Meetings 2016. Austin, TX.


2016 Schield, D.R.†, R.H. Adams, D.C. Card, B.W. Perry, T. Jezkova, A.L. Andrew, G.I. Pasquesi, S. Mackessy, & T.A. Castoe. Patterns of speciation and delimitations of populations, species, and genomic adaptations across widely-distributed North American rattlesnake lineages. Joint Evolution Meetings 2016. Austin, TX.


2016 Streicher, J.W.†, J. McEntee, L. Drzich, D.C. Card, D.R. Schield, U. Smart, C. Parkinson, T. Jezkova, E.N. Snith, & T.A. Castoe. Genetic surfing, not allopatric divergence, explains spatial sorting of mitochondrial haplotypes in venomous coralsnakes. Joint Evolution Meetings 2016. Austin, TX.


2016 Adams, R.H.†, D.R. Schield, D.C. Card, & T.A. Castoe. Friend or Foe: Investigating the impacts of positive selection on multispecies coalescent inferences. Joint Evolution Meetings 2016. Austin, TX.


2016 Jezkova, T.†, T.A. Castoe, M. Leal, D.C Card, D.R. Schield, D. Elzinga, & J. Rodriguez-Robles. A peculiar case of hybridization with advantageous mtDNA introgression and lack of nuclear introgression in Caribbean anoles. Joint Evolution Meetings 2016. Austin, TX.


2016 Pasquesi, G.I.†, D.C. Card, R.H. Adams, A.B. Corbin, D.R. Schield, & T.A. Castoe. Evolutionary dynamics of genomic repeat element landscapes across 200 million years of squamate evolution. Joint Evolution Meetings 2016. Austin, TX.


2016 Castoe, T.A.†, G.I. Pasquesi, R.H. Adams, D.C. Card, & D.R. Schield. Sample sequencing 68 squamate genomes reveals extensive genomic repeat landscape dynamics. International Congress on Transposable Elements. Saint Malo, France.


2016 Card, D.C.†, D.R. Schield, R.H. Adams, M. Hunter, K. Hart, & T.A. Castoe. Genome-wide evidence of evolution and adaptation in the invasive Florida python population. Annual Celebration of Excellence by Students (UTA-ACES). Arlington, TX.


2016 Adams, R.H.†, D.R. Schield, D.C. Card, & T.A. Castoe. Natural selection and multispecies coalescent models: Investigating the impacts of directional selection on phylogenetic inferences of recently-diverged species complexes. Annual Celebration of Excellence by Students (UTA-ACES). Arlington, TX.


2016 Andrew, A.L.†, D.C Card, D.R. Schield, B.W. Perry, R.H. Adams, R.P. Ruggiero, D.D. Pollock, S.M. Secor, & T.A Castoe. Dynamics of gene expression and mechanisms of growth throughout post-prandial organ regeneration in the Burmese python. Annual Celebration of Excellence by Students (UTA-ACES). Arlington, TX.


2016 Pasquesi, G.I.†, D.C. Card, R.H. Adams, D.R. Schield, A.B. Corbin, B.W. Perry, A.L. Andrew, & T.A. Castoe. Sample sequencing 68 squamate genome reveals extensive evolutionary dynamics of genomics repeat element landscapes. Annual Celebration of Excellence by Students (UTA-ACES). Arlington, TX.


2015 Reyes-Velasco, J.†, D.C. Card, A.L. Andrew, K.J. Shaney, R.H. Adams, D.R. Schield, N.R. Casewell, S.P. Mackessy, & T.A. Castoe. Expression of venom gene homologs in diverse python tissues: a new model for the evolution of snake venom and a re-assignment of transcriptome-based definitions of venoms. International Society on Toxinology, 18th World Contress. Oxford, England.


2015 Card, D.C.†, D.R. Schield, R.H. Adams, W. Booth, S. Boback, & T.A. Castoe. Mitochondrial and nuclear SNP evidence for population genetic structure, independent islands dwarfism, and genes putatively underlying dwarfism traits in Boa constrictor. Society for the Study of Amphibians and Reptiles Meeting. Lawrence, KS.


2015 Castoe, T.A.†, D.C. Card, D.R. Schield, J. Reyes-Velasco, A.L. Andrew, R.H. Adams, & G.I. Pasquesi. Ancient, recent, and ongoing patterns of genome evlution underlying adaptation and innovation in snakes. Society for the Study of Amphibians and Reptiles Meeting. Lawrence, KS.


2015 Andrew, A.L.†, D.C. Card, R.P. Ruggiero, D.R. Schield, R.H. Adams, D.D. Pollock, S.M. Secor, E. La, & T.A. Castoe. Patterns of gene expression underlying extreme physiological remodeling in snakes. Society for the Study of Amphibians and Reptiles Meeting. Lawrence, KS.


2015 Schield, D.R.†, G.I. Pasquesi, R.H. Adams, D.C. Card, & T.A. Castoe. Patterns of selection and introgression following isolation and secondary contact in a widely-distributed rattlesnake species (Crotalus atrox). Society for the Study of Amphibians and Reptiles Meeting. Lawrence, KS.


2015 Adams, R.H.†, D.R. Schield, D.C. Card, S.P. Mackessy, & T.A. Castoe. How does natural selection influence species trees and species delimintation using genome-scale datasets - a case study in rattlesnakes. Society for the Study of Amphibians and Reptiles Meeting. Lawrence, KS.


2015 Card, D.C.†, D.R. Schield, K. Hart, M.E. Hunter, & T.A. Castoe. Evolution and putative adaptation in the invasive Florida Burmese python population inferred using genome-wide RADseq data. Society for Molecular Biology and Evolution Meeting 2015. Vienna, Austria.


2015 Card, D.C.†, D.R. Schield, R.H. Adams, W. Booth, S.M. Boback, & T.A. Castoe. Genomic basis of adaptive island dwarfism in Boa constrictor. Annual Celebration of Excellence by Students (UTA-ACES). Arlington, TX.


2015 Schield, D.R.†, M.R. Walsh, D.C. Card, A.L. Andrew, R.H. Adams, & T.A. Castoe. EpiRADseq: a novel technique for the examination of genome-wide epigenetic patterns. Annual Celebration of Excellence by Students (UTA-ACES). Arlington, TX.


2015 Adams, R.H.†, D.C. Card, J. Reyes-Velasco, D.R. Schield, A.L. Andrew, & T.A. Castoe. Sample sequencing 46 squamate reptiles reveals extensive evolutionary dynamics of genomic repeat landscapes. Annual Celebration of Excellence by Students (UTA-ACES). Arlington, TX.


2015 Andrew, A.L.†, D.C. Card, R.P. Ruggiero, D.R. Schield, R.H. Adams, D.D. Pollock, S.M. Secor, E. La*, & T.A. Castoe. Patterns of gene expression underlying extreme physiological remodeling in snakes. Annual Celebration of Excellence by Students (UTA-ACES). Arlington, TX.



Service

Vice President – Phi Sigma Biological Honor Society, Beta Phi chapter (2013 – present)

Session chair - Joint Evolution Meetings 2014, Raleigh, NC

Session chair - Joint Evolution Meetings 2016, Austin, TX

Ad hoc reviewer for Evolution, PLoS One, Toxicon, and Phyllomedusa


Professional Societies

American Academy of Arts & Sciences

Society for the Study of Evolution

Society of Systematic Biologists

Society for the Study of Amphibians and Reptiles

Snake Genomics Consortium


Awards and Honors

2015NSF Doctoral Dissertation Improvement Grant ($19,695)

2015Research Grant – Phi Sigma Biological Sciences Honor Society ($5000)

2013Research Grant – Phi Sigma Biological Sciences Honor Society ($1250)

2013Travel Award – Evolution meeting, Snowbird, UT. ($500)

2013Travel Award – Mechanisms of Protein Evolution II meeting, Denver, CO. ($500)

2012Enhanced Graduate Teaching Fellowship – UTA (5 years)

2012Jay Y. Roshal Award – University of Minnesota Morris ($500)

2011Undergraduate Research Opportunity Program Award – University of Minnesota Morris ($1,700)

2010Morris Student Administrative Fellowship – University of Minnesota Morris

(5 semesters, $500 per semester)


Teaching Experience

University of Texas at Arlington, Arlington, TX

Biology 1442: Structure and Function of Organisms laboratory (8 semesters)


Tutor (9/2013 – present)

University of Texas at Arlington, Arlington, TX

Genetics


Contact information

Office – ERB 444

Email – dschield@uta.edu


 

WHAT WE DO IN  THE LAB
Evolutionary Genomics

Reptiles, esp. Snake Genomics

Genomics of Extreme Adaptations

Systems Biology

Population Genomics

UTA LINKS

   UTA

        Biology Department

            Graduate Admissions

            Genome Biology Group

            Amphibian and Reptile

            Diversity Research Center

Drew R. Schield

Quantitative Biology, Ph.D. Student


Origins

Twin Cities, Minnesota, USA


Degrees

B.A. – Biology, University of Minnesota, Morris 2012


Research

I am interested in speciation and adaptation in natural populations, and investigating these processes using genomic resources. More specifically, I am interested in the paradoxical phenomenon of speciation with gene flow, and understanding how selection on genomic regions may act to maintain lineage diversity in the face of gene flow, and how the intersection of the processes of divergence and admixture relate to biodiversity.